Training on commercial mushroom Propagation: Shitake mushroom Lentinula edodes as a model
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Cultivation of Shiitake mushroom, Lentinula edodes, Small and medium incubators.
Abstract
For the purpose of training on commercial propagation of mushroom mycelium, a biopsy of Shiitake mushroom Lentinula edodes was taken from the bottom of the cap and injected into PDA Potato Dextrose Agar in Petri dishes which were then incubated at 24±0.1C. After 3 days of injection, growth of the mushroom mycelium began to spread. After 7 days, a halo of mycelium was formed around the tissue biopsy. After 20 days, growth of the mushroom mycelium was completed on the whole of the dish. Due to the fact, commercial form of mycelium which is used in mushroom farms involves sterilized cereal seeds, Mycelium of every Petri dish was divided into 8 discs which were then mixed with sterilized wheat seeds in flasks and incubated at 24±0.1C. Shiitake mushroom mycelium began to emerge and spread after 7 days, spread clearly within 14 days and colonized the wheat seeds completely after 21 days. The seeds were then ready to be used for inoculation of compost in mushroom farms.
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