Journal of Medical Sciences 2022-06-06T09:58:11+00:00 Prof . Dr . Ibrahim Abu Shanaf Open Journal Systems <p>Excellence in publishing scientific production, according to methodological standards and ethics of academic research. And contribute to the enrichment of all areas of science and specialized knowledge; accurately, honestly, and objective. And advance the level of scientific research in cooperation with the corresponding specialized scientific bodies and institutions, wherever they are. And keeping pace with scientific development and its techniques, to contribute to the development of the local community, and meet its knowledge needs. And increase scientific production, and publish theoretical research and applied studies, in order to achieve leadership at the national level. And provide opportunities for creativity and distinction for faculty and academics, by raising their academic degrees and professional competence. And work to make the scientific journal in the university a center of knowledge radiation at the local, national and international levels.</p> Genotyping of Hepatitis C Virus isolated from Libyan patients by line immune Probe Assay ( LiPA ) 2022-05-30T09:34:12+00:00 Fatma I. E. Ali Najem Suliman A. Elgadi Ibrahim A. Taher Halema S. Ali Amina .S. Abusedra <p>Chronic HCV infection contributed with the development of some diseases such as liver cirrhosis, cancer, and liver failure especially in HIV-positive patients during active antiretroviral therapy which may lead to death. This was the reason that genotyping has become a very important in routine diagnosis and management of chronic viral hepatitis infections. The purpose of this study was to see HCV genotypes among Libyan patients by LiPA technique, were a 75 blood samples were collected&nbsp; and&nbsp; processed at the laboratory of BIDIC (Benghazi Center of Infectious disease and Immunity) to perform the viral load and HCV genotyping. It was found that (60 %) of the collected samples belongs to HCV genotype 4, and (26.7 %) subtype 1a, while other subtypes 2a/2c, subtype 1b; Subtype&nbsp;&nbsp;&nbsp; 1a /1b; and genotype 2 all represented 5.3%; 5.3 %; 1.3 %; 1.3 % respectively. This concluded that HCVgenotypes in libya is distributed consuistently with Libyan neighboring countries. And the analysis of the 5′ UTR by LiPA with the INNO-LiPA HCV II kit can provide a quick, simple and reliable method for determination of the HCV genotype.</p> 2022-05-30T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences Evaluation Of Community Knowledge About Awareness And Risk Factors For Stroke Disease In Benghazi, Libya 2022-06-06T09:58:11+00:00 Hana A. Habib Saad Mailoda A. Hassan Noha H. Hammed Heba A. Yonis Heba S. Daihoum <p>Stroke is an important cause of disability among adults and is one of the leading causes of death worldwide. Stroke is a term used to describe an abrupt-onset focal neurologic deficit that lasts at least 24 hours and is of presumed vascular origin[1]. Stroke can be either ischemic or hemorrhagic (88% and 12% respectively, of all strokes in the 2003 American Heart Association report[2], Risk factors for stroke can be subdivided into non-modifiable and modifiable[3]. Aim: this study aims to investigate community knowledge about stroke and its risk factors. Method and Procedures: A cross-sectional observational study was conducted in polyclinics located in Benghazi between November to January 2017-2018. Once before the interview, participants were asked to verbal consent to participate in the study. Participants were interviewed using pre-piloted questionnaire and their history of their morbidity and their lifestyle. Results During the study period, a total of 229 people, the response rate was (93%). The majority was in the younger age group between (18-29-year-old) found 84 (37%), female 153 (67%) and high education people 138(60%). In current study the information source most of friends 90 (40%), less percentage internet 23 (10%) and work shop 23 (10%). Conclusion: The current study shows that the most of participants can define the disease but their knowledge about its risk factors and dangerous signs were not enough. Almost of participants depend on their friends as source of information.</p> 2022-06-06T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences Maxillary crossbite correction with a rapid palatal expansion followed by a corrective orthodontic treatment 2022-05-16T09:06:41+00:00 Atef Omar Abosalah Eissa Ahmed Omar Abousalah Eassa Abdulsalam. E.E. Ibrahim <p>This case report presents the&nbsp; orthodontic treatment of a Libyan girl&nbsp; , aged 14 years 4 months with a Class lll&nbsp; malocclusion with severe transverse maxillary deficiency ( anterior sever crowding ,canines buccally eruption&nbsp; and posterior bilateral&nbsp; crossbite)(Figure 1) and correction using Haas expander and fixed orthodontic appliance(Figure2). The treatment goals were to correct the posterior crossbite and anterior crowding and restore the normality of the dentition and occlusion. In phase I, the patient was treated with a modified Haas-type palatal expander, which provided a clinically significant palatal expansion and increased the maxillary arch perimeter with favourable conditions for orthodontic treatment with fixed appliances in phase II. A removable wraparound type appliance and a bonded lingual canine-to-canine retainer were used as retention. Although the literature has reported a high rate of relapse after palatal expansion, after 2 years 9 months of post treatment follow-up, the occlusal result was stable and no skeletal reversals could be detected.</p> 2022-05-16T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences Cancer Testis Antigen (CTA) T21, as a Potential Diagnostic, Prognostic, and Immune-Therapeutic Targets for Malignant Tumours. 2022-05-10T09:07:36+00:00 Hoda M Tawel Yousef M Ali Hasen Amanda Miles <p>CTAs, are a family of tumour-specific shared antigens that represent promising targets for cancer immunotherapy, as well as, diagnostic and prognostic markers for tumour development.&nbsp;&nbsp;&nbsp; T21 is a novel antigen with little information known about its protein expression in malignant cells.&nbsp; This study was aimed at evaluating the expression of T21 antigen as a potential diagnostic and prognostic marker for diverse malignancies.&nbsp;&nbsp; Material and methods: IHC staining was applied using a mono-specific polyclonal antibody against T21 to diverse paraffin-embedded malignant tissue microarrays. The specificity of the staining was confirmed by the negativity of the isotype controls. The slides were imaged to visualise the positive T21 staining using an inverted light microscope (x 10) with digital net camera.&nbsp; IBM-SPSS statistic software, version 22, was used for descriptive and statistical data analysis.&nbsp; Results: T21 was expressed in a large percentage of the examined primary (84.9%) and metastatic (65%) tumour sections. T21 expression was evident in ccRCC, adenocarcinoma of small intestine, testicular tumours, adenocarcinoma of rectum, squamous cell carcinoma of oesophagus and astrocytoma. For metastatic tumours, the expression was remarkable in metastatic squamous cell carcinoma of unknown origin, metastatic breast carcinoma and metastatic thyroid carcinoma. There was no correlation the between T21 expression and tumour staging.&nbsp; Conclusion: T21 represents a potential target for cancer immunotherapy and vaccination.&nbsp; Overexpression of T21 antigen represents a useful diagnostic biomarker for cancer progression, but caution should be used when considering its potential for use as a prognostic marker. Further inclusive work focusing on the correlation of T21 expression and tumour staging is needed.</p> 2022-01-30T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences A comparision between blood glucose concentration measured in capillary and venous blood using glucometer and measurements in venous plasma using Auto analyser 2022-06-06T09:22:00+00:00 Tahani Y. Omar <p>There are several methods for laboratory measurement of blood glucose. The detection, identification and quantification of glucose in blood has played a vital role in the diagnosis and management of patients suffering from disorders of carbohydrate metabolism and is one of the most frequently performed determinations in clinical chemistry. Aim: The purpose of this study is to&nbsp; the Compar between&nbsp; different&nbsp; methods of&nbsp;&nbsp; blood&nbsp; glucose estimations in&nbsp; venous whole blood,&nbsp; capillary whole blood by glucometer, and plasma glucose estimation by Auto analyser. Materials and Methods:&nbsp; A&nbsp; 60 adult patients were randomly selected and included in the study, to measure the blood glucose. Blood samples were collected from all persons (fasting 10/12-hour overnight), whether a known case of diabetes or not. Venous whole blood glucose, Capillary whole (Finger prick)&nbsp; blood glucose estimation was done by glucometer; and venous plasma glucose estimation was done by auto analyzer in laboratory. Results:&nbsp;&nbsp; Means of Venous Whole blood glucose level measurements and finger-prick blood glucose level measurements&nbsp; were the highest, 146.63±10.16 mg/dL and 143.80±9.73 mg/dL, respectively, which indicates that the means were higher than that of Venous plasma glucose level measurements, but this rise was statistically insignificant when compared to the Venous plasma glucose level measurements 120.67±8.88 mg/dL. The percentage statistically insignificant elevations of blood glucose concentrations in capillary whole blood and venous Whole blood were 19.17 % and&nbsp; 21.51% respectively when compared with the control value( plasma blood glucose). Conclusion: Capillary blood glucose estimation by glucometer is a faster method for glucose estimation , provided that the device used for the measurement is calibrated according to the manufacturer's conditions, but the main controversial issue is the reliability of glucometers, as they may show large deviations. However, a degree of caution should be exercised in the interpretation of bedside glucometer measurements as they may not be sufficiently accurate to replace laboratory blood glucose results.As the study includes 60 sampl only, a larger study is necessary for authentication of the findings. Although, many studies also show the same results. Future research with a larger sample size should be done.</p> 2022-06-06T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences Head lice(Pediculus humanus capitis) infestation and the role of health education in limiting its spread among children at Primary-School in Misurata,Libya 2022-05-16T10:05:01+00:00 Naima Hussin Ftattet Fatema Ahmad Gloos <p>Pediculus humanus capitisis a worldwide scourge and children are more susceptible to infection than adults. Therefore, implementing health education programmes to control the spread of Head lice requires a great effort from teachers and parents. This study was conducted from November 2019 to February 2020 at some primary schools in Misurata city. The study community included 825 (male &amp; female) students between the ages of 7and 12. A first test was conducted to detect the prevalence of the infection with Pediculus humanus capitisis among students and the main influencing risk factors. Another test was conducted to achieve the effectiveness of health education in reducing infection . The results of this study revealed that the number of children with Pediculus humanus capitisis was 197 i.e. (23.9%) out of the 825 examined students. This percentage has decreased to (11.5%) following the intervention of health education programmes, with a statistical significance of (P&lt;0.05). Among the factors, the study showed two significant correlations(P&lt;0.05) . The first was between the infection rate and the participants’sex as the highest rate of infection was recorded among females at (31.1%). The other significant correlation was between the infection rate and the age, with the highest rate reaching (33.9%)among those aged 7years. It is concluded that the infection with Pediculus humanus capitisis has increased among primary-school students in Misurata when compared to other related studies. However, health awareness programmes at schools proved to have a beneficial effect in preventing and controlling the infection with Pediculus humanus capitisis.</p> 2022-05-16T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences Study of the prevalence of malaria parasite in migrant workers using rapid diagnostic test (RDTs) in Sebha, Libya. 2022-05-10T10:50:41+00:00 Naima I. Alhaddad Rugaia M. A. El-Salem <p>Malaria is a parasitic infection caused by a parasite of the Plasmodiidae family, Plasmodium that infects humans and is transmitted by female Anopheles mosquitoes. 229 million cases of malaria were recorded worldwide in 2019 and caused 409,000 deaths. Africa bears the brunt of this Infections Especially in sub-Saharan Africa, imported malaria cases among expatriates coming from endemic areas to malaria-free countries are a risk factor for these countries, so testing RDTs for the detection of the parasite in this category has been used as one of the prevention and control programs for the disease that requires intensification by Several parties related to disease control. This study was conducted during February and March of the year 2020 in the health care center in Sebha city. The study population included 135 people (males and females), their ages ranged from 15-67 years, who were subjected to a standardized questionnaire and examination of venous blood samples using the RDTs test.</p> <p>The results of our study showed that the prevalence rate of 14.8% (20) malaria cases out of the total number of cases, the age group 15-24 males had the highest incidence of Plasmodium falciparum malaria, and it was found through our study that there is a significant correlation between infection rate and educational level P=0.00 This is due to the lack of knowledge of ways to prevent the disease, and there was a significant correlation between infection rate and housing, P=0.02, which confirms the presence of a high prevalence of the vector in the affected villages. The aim of the study is to determine the extent of the malaria parasite prevalence and associated risk factors among expatriate workers in the city of Sebha using the Rapid Diagnostic Test (RDTs).</p> 2022-01-30T00:00:00+00:00 Copyright (c) 2022 Journal of Medical Sciences