Sebha University Conference Proceedings

Isolation and identification of two strains of mercury resistant fungi Aspergillus terreusus and Fusarium solani from soil.

2024-08-01T12:42:00+02:00

The current study included isolating and identifying the fungi Aspergillus terreusus and Fusarium solani and testing their growth at different concentrations of mercury. An atomic absorption spectrometer was used to estimate mercury before and after inoculation with the isolated fungi. Several concentrations of the metal were used (25, 50, 100, 125, 150). 200 and 300 milligrams per liter) the isolated fungi were able to grow at high concentrations (200 and 300 milligrams per liter). The results showed a decrease in the rate of the tested concentrations after inoculation and incubation for seven days at a temperature of 28°C, as the highest value of mercury absorption was reached at a concentration of 125 mg/L for F. solani after the aforementioned incubation period. Through the study, it was found that the isolated fungi were similar in terms of reducing the concentration of the metal under study. Accordingly, the current study indicates that the isolated fungi can be used as biological treatment agents for soil polluted with mercury.

A qualitative study of agricultural wet soil mosses in some areas of the city of Ubari (southern Libya).

2024-08-11T12:24:59+02:00

This study was conducted to identify and determine what types of algae may be found in the wet soil in the agricultural fields of some areas of Ubari, and to know the role of algae in soil fertility, this study aimed to identify and determine to measure some of the physical and chemical factors of the soil, diagnose the algae present in it, and determine the relationship between them statistically. At three locations in the Ubari regions, represented by (Al-Ghuraifeh - Ibrik - Qaraqra), with two farms in each region, the samples were collected during the fall semester of 2022. As for the chemical factors, the value of nitrate ranged (8.51-31.30 mg/L), the value of calcium ranged between (3.50-10) mg/L, magnesium (0.30-8.90) mg/L, and the values of both sodium and potassium ranged between (32.80-205.30) mg/L (0.30 – 28.30) mg/L respectively. The number of algae species identified in the current study reached 37 species, with Cyanophyta predominant at 43.2%, followed by Chlorophyta at 32.43%, then Bacillariophyta at 10.8%, Xanthophyta at 5.4%, and Euglenophyta, Dinophyta, and Phaeophyta at 2.7%. Nitella aemula A is considered to be strong among genera throughout regions. Braun was the stronger species in Chlorophyta and was present in both the second site's first field (M.B.H.1) and the third site's second field (M.C.H.2). In relation to Cyanophyta, Microcystis stagnalis was the most common species and was present in all study sites.

Determine the association of antibiotic resistance with the extra chromosome

2024-08-11T13:31:47+02:00

ntibiotic resistance is a major public health problem. And that the spread of resistance genes and the lack of effective antibiotics exacerbates the problem, and that when resistance genes are present on plasmids, they are responsible for the spread of antibiotic resistance genes, Genetic transformation is a method of transmitting plasmids. This study aims to determine the association of antibiotic resistance with the extra chromosome. The bacteria was isolates of Gram-negative bacteria represented by E.coli. Sensitivity test of the bacterium against antibiotics was established, To study the role of plasmids in antibiotics resistance, curing experiment was using Ethidium Bromide (Et.Br), Sodium dodecyl sulfate. The results showed bacteria to resistant antibiotics of the Phenicol،Cephalosporin، Tetracycline، Glycopeptide، Macrolid، Polymyxins، β- Lactamase, AND sensitive to the OTHER ANTIBIOTIC, and were detected high sensitive to fluoroquinolones, Nitrofuran. The results of the molecular genetic content assay showed of the bacteria E.coli9 that contained three plasmid bundles, and E.coli12 contained a bundle of plasmid in addition to a large plasmid, Results showed the loss of resistance of the antibiotic Erythromycin and Chloramphenicol, and that resistance can be transmitted by plasmids by horizontal gene transfer (genetic transformation). Thus, antibiotic resistance genes can be acquired .The plasmid bears antibiotic resistance.

Isolation of bacteria from various types of raw milk and assessing their antibiotic sensitivity in the city of Sabha.

2024-08-13T12:29:34+02:00

This study focuses on the analysis and isolation of bacteria from various types of raw milk and testing their sensitivity to antibiotics in the city of Sabha. The primary objective of the research reflects its potential conclusions based on raw milk samples, with a specific focus on potential sources of contamination and the evaluation of milk quality, particularly concerning the presence of Listeria monocytogenes bacteria. The study aims to better understand the potential risks that may impact the dairy industry and its products, and to provide scientific foundations for the development of effective strategies for monitoring, prevention of contamination, and ensuring the safety of food products.The study analyzed samples of raw milk from different areas of Sabha over a specified period, with the aim of identifying present bacteria and assessing their sensitivity to antibiotics. It was found that there is a potential contamination in milk with high levels of certain bacteria such as Escherichia coli and Listeria monocytogenes, necessitating health measures to control it. Furthermore. were collected 25 samples of raw milk from different stores within the city of Sabha, including stores in Al-Manshiya, Al-Qarada, and Al-Jadid. The samples were distributed as follows: 7 samples of camel milk, 10 samples of cow milk, and 8 samples of goat milk. After using microbial diagnosis, bacteria were obtained: Salmonella spp, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Klebsiella spp, Pseudomonas spp, Enterobacter spp A test was conducted for the sensitivity of bacteria to antibiotics. It was found that all bacterial species were 85% resistant to antibiotics. The p value was approximately (\(7.42 \times 10^{-16}\)،) and this confirms the presence of significant differences between the rates of resistance to antibiotics. The study revealed high levels of bacterial resistance to antibiotics, highlighting the need for specific monitoring strategies and the development of policies to combat this issue. Overall, this study provides valuable insights into milk contamination and antibiotic resistance, emphasizing the importance of cleanliness and control in the dairy industry to ensure the safety of food products and the development of appropriate health policies.

In vitro Culture of lateral buds and zygotic embryos of Date Palm trees (Phoenix dactylifera) Talis (CVs).

2024-08-13T12:40:04+02:00

The lateral buds and zygotic embryos of date palm (Phoenix dactylifera var Talus) were cultured on different concentrations of plant hormones (2,4-D, NAA, IAA, Kin, and adenine) and activated charcoal (AC). Results revealed that the best responses were recorded when using MS + 2,4-D (20 mg/l), MS + IAA (20 mg/l) + AC (3 g/l) as high amounts of white granular friable culture after 9 weeks. Small amounts of callus were formed after one week, then small branches were regenerated and reached 3 centimeters in length after four weeks, respectively. When they inoculated on MS+ 2,4-D(20mg/l+Ad(5mg/l) +AC(3g/l) callus was induced after 1 week and small globular buds were formed after 2 weeks. No observations were recorded in the other treatment cases. The growth of callus increased when subculture formed MS + IAA (20 mg/l) + AC (3 g/l) to MS + Adenine (5 mg/l) + AC (3 g/l) after a week. On the other hand, no responses were noticed in the other treatments. When zygotic embryos were cultured on MS + 2,4-D (0.5 mg/l) + BAP (0.5 mg/l), only an increase in their growth after 2 weeks and a change in their color in the case of another treatment.

Studing the effect of hypothyroidism on pregnancy in female rats

2024-08-13T12:50:53+02:00

Thirty albino Wistar rats were used in this research to investigate the effect of the hypothyroidism-inducing drug propylthiouracil on the pregnancy function and phenotypes of rat offspring, including 10 males for mating only, and 20 females were divided into two groups, namely control and treated, and the stages of the estrous cycle of females were determined by vaginal swab test. The stages of the estrous cycle of females were determined by vaginal smear test, and experimental hypothyroidism was induced with propylthiouracil at a concentration of 10 mg/kg for 30 days by oral cavage. The pregnancy period and number of pups were recorded in both groups and the weights of the pups were taken during a 4-week period using a sensitive scale. The results of this study showed a clear appearance of nucleated and keratinised epithelial cells during the proestrus phase compared to the estrus phase, where a number of large nucleated cells were observed with a high number of keratinised epithelial cells. The phenotypic results of rats showed non-significant differences in the pregnancy period and the number of pups, while the drug achieved negative results on the rats by reducing the weight of the pups in the treatment group 24.93±88 compared to the control.

ON BI-TOPOLOGICAL SPACE

2024-08-13T12:56:00+02:00

Bi-topological spaces, a generalization of traditional topological spaces, provide a rich framework for studying the interplay between two distinct topologies defined on a single set. In a bi-topological space, open sets and neighborhoods are characterized by two separate systems of open sets, offering a nuanced understanding of continuity, convergence, and compactness properties. This abstract explores the foundational concepts of bi-topological spaces, including their definition, basic properties, and key theorems. Moreover, it discusses the significance of bi-topological spaces in various mathematical contexts, highlighting their applications in fields such as functional analysis, differential equations, and computer science. Through the lens of bi-topological spaces, these abstract illuminates the versatility and relevance of this mathematical structure in both theoretical investigations and practical applications.