Anatomical Study of Leaves of Tragnum nudatum by plant tissues culture technique
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Anatomica, Callus, Leaves, Tragnum
Abstract
The Segments of leaves of Tragnum nudatum were Cultured on MS medium supplemented with different concentrations of( 2,4-D,BA,Kin)in order to study antomy of the explants after2,4,6,8,10,12 weeks. The study revealed that parenchyma cells with thin cell wall and rectangular shape ,tracheids and Sclerenchyma. The best responses were recorded in the case of using MS + Kin (1mg/l) as meristmtaic cells were scattered through sections after 10 weeks. When the explants we Culture on MS + (1mg/l)+2,4-D meristamtic cells were formed in groups in 20% and meristmatic centers were observed after 10 weeks of culture.
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