Detection of the presence of residual antibiotics commonly used in fresh cow's milk produced in some regions of North Western of Libya.
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Abstract
This study was conducted at the Food and Drug Control Center, Al-Falah Branch / Tripoli, to investigate the presence in milk of residual antibiotics commonly used in local dairy farms. The study took a period from May to August (2021). in which a hundred fresh cow’s milk samples were randomly collected from five different regions Libya (Al-Zawiya - Ghout Al-Rumman - Al-Najilah - Tajoura - Al-Sawani) at the rate of twenty samples per region. Knowing that the cows were fed concentrate (cow concentrate) and the prevailing natural pastures in their regions. The high-performance liquid chromatography device examined the milk samples with a UV detector and an isocratic method. The samples were subjected to solid phase extraction before analysis and a mobile phase consisting of methanol, acetonitrile, oxalic acid 0.01 mol/L (5%, 18%, 77%), with a flow rate of (0.7 ml/min). The results of the study were analyzed based on the statistical package program for social sciences (SPSS Statistics 20) and using the percentage (the number of samples containing antibiotic residue was calculated against the total number of samples examined as a percentage), and the Student t-test (T) to compare the averages for positive samples and the permissible limit for tetracycline internationally. The results showed that 4 (4%) out of 100 milk samples contained residues of the antibiotic (chlortetracycline), with an average total concentration of 68.85 ± 9.8 µg/L. 2 (50%) positive were samples from Al-Zawiya area and 2 (50%) positive were samples from Ghut Al-Rumman area
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References
.الكتاب السنوي للإحصاءات الزراعية العربية، ( 2019).
Nguyen Bao, K. L., Sandjaja, S., Poh, B. K., Rojroongwasinkul, N., Huu, C. N., Sumedi, E., and SEANUTS Study Group. (2018). The consumption of dairy and its association with nutritional status in the South East Asian Nutrition Surveys (SEANUTS). Nutrients, 10(6), 759.
Darwish, W.S., Eldaly, E.A., El-Abbasy, M.T., Ikenaka, Y., Nakayama, S. and Ishizuka, M. (2013). Antibiotic residues in food: The African scenario. Japanese Journal of Veterinary Research, 61(Supplement S13-S22.
Van Boeckel, T.P., Brower, C., Gilbert, M., Grenfell, B.T. and Levin, S., A, Robinson TP, Teillant A, Laxminarayan R(2015). Global Trends in Antimicrobial Use in Food Animals. Proc. Natl. Acad. Sci, 112, Pp.5649-5654.
Lupin, H. M. (2009). Human health aspects of drug and chemical use in aquaculture. The Use of Veterinary Drugs and Vaccines in Mediterranean Aquaculture, 95-103.
Taverniers, I., De Loose, M. and Van Bockstaele, E. (2004). Trends in Quality in the Analytical Laboratory. II. Analytical Method Validation and Quality Assurance. Trac Trends in Analytical Chemistry, 23(8):535-552.
Alnassrallah, M. N., Alzoman, N. Z., and Almomen, A. (2022). Qualitative immunoassay for the determination of tetracycline antibiotic residues in milk samples followed by a quantitative improved HPLC-DAD method. Scientific reports, 12(1): 1-14.
Das, Y. K., Yavuz, O., Atmaca, E., and Aksoy, A. (2019). Tetracycline antibiotics in raw cow’s milk produced in Samsun Province, Turkey. Fresenius Environ. Bull, 28, 5982-5988.
Dawn Merton Boothe (2015). The Merck Veterinary Manual. Merck sharp & dohme corp., subsidiary of Merck &co., lnc., kenil worth,N.J.,U.S.A.
Bando, E., Oliveira, R.C., Ferreira, G.M.Z. and Machinski Jr, M. (2009). Occurrence of antimicrobial residues in pasteurized milk commercialized in the state of Paraná, Brazil. Journal of food protection, 72(4): 911-914.
Zanella, G.N., Mikcha, J.M.G., Bando, E., Siqueira, V.L.D. and Machinski Jr, M(2010). Occurrence and Antibiotic Resistance of Coliform Bacteria and Antimicrobial Residues in Pasteurized Cow's Milk from Brazil. Journal of Food Protection, 73(9):1684-1687.
Al Zuheir, I. M. (2012). Detection of β-lactams and tetracyclines antimicrobial residues in raw dairy milk for human consumption in Palestine. Walailak Journal of Science and Technology (WJST), 9(3): 277-279.
Food and Drug Administration. (2015). Milk drug residue sampling survey. Silver Spring, 10. WHO(2015) (2017) (2018). Antibiotic Resistance: Multi-Country Public Awareness Survey. Report No.: 9241509813. Food and Agriculture Organization of the United Nations .(2019). Gateway to dairy production and products.