Evaluation of four commercial COVID-19 diagnostic RT-PCR kits used in Libya

Authors

  • Khadija M. Ahmad  Department of Microbiology, faculty of medicine, Sebha University, 
  • Shamsi S Shamsi  Department of Plant Science, Microbiology Division, Research Laboratory, Sebha University
  • Mohammed B Alsubbi General Department, Faculty of Nursing, Sebha University, Libya
  • Ali A. Aldalouli Department, PCR unit, clinical laboratory, Sebha Medical center.
  • Hamad A Almahdi Department, PCR unit, clinical laboratory, Sebha Medical center
  • Alkeelani M M Alkeelani Department, PCR unit, clinical laboratory, Sebha Medical center
  • Ibrahim A. Mohamed Department of Biochemistry, faculty of medicine, Sebha University.

DOI:

https://doi.org/10.51984/joms.v18i1.2600

Keywords:

Real-time PCR, COVID-19, molecular technique, respiratory infection, diagnostic kits

Abstract

Background: Real-time reverse transcriptase chain reaction is considered the most sensitive and specific assay for COVID-19 detection. This study was planned to compare and evaluate four commercially diagnostic kits. Material and methods: 92 Nasopharyngeal swabs collected from different symptomatic patients were studied in this project. The total nucleic acid (RNA) was extracted, and RT-PCR was done using four commercial diagnostic kits from different manufacturers, DANN and BGI (China), Hibrigen, and Bio-speedy (Turkey). Result: Although all 92 clinical samples were subjected to the same four diagnostic kits, the results revealed variations in the performance. The total number of positive cases was (42/92) for Da An Gene, BGI (36/92), Bio-speedy (31/92), and Hibrirgen (14/92).

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Author Biographies

  • Khadija M. Ahmad,  Department of Microbiology, faculty of medicine, Sebha University, 

     Department of Microbiology, faculty of medicine, Sebha University, 

  • Shamsi S Shamsi,  Department of Plant Science, Microbiology Division, Research Laboratory, Sebha University

     Department of Plant Science, Microbiology Division, Research Laboratory, Sebha University

  • Mohammed B Alsubbi, General Department, Faculty of Nursing, Sebha University, Libya

    General Department, Faculty of Nursing, Sebha University, Libya

  • Ali A. Aldalouli, Department, PCR unit, clinical laboratory, Sebha Medical center.

    Department, PCR unit, clinical laboratory, Sebha Medical center

  • Hamad A Almahdi, Department, PCR unit, clinical laboratory, Sebha Medical center

    Department, PCR unit, clinical laboratory, Sebha Medical center

  • Alkeelani M M Alkeelani, Department, PCR unit, clinical laboratory, Sebha Medical center

    Department, PCR unit, clinical laboratory, Sebha Medical center

  • Ibrahim A. Mohamed, Department of Biochemistry, faculty of medicine, Sebha University.

    Department of Biochemistry, faculty of medicine, Sebha University.

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Published

2023-05-20

Issue

Vol. 18 No. 1 (2023): Exploring Diverse Research in Science and Healthcare: Insights from Our Latest Issue

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Copyright (c) 2023 Journal of Medical Sciences

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How to Cite

Evaluation of four commercial COVID-19 diagnostic RT-PCR kits used in Libya. (2023). Journal of Medical Sciences, 18(1), 26-30. https://doi.org/10.51984/joms.v18i1.2600

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