Evaluation of Genotoxicity of Acetone Extract of Dodonaea Viscose L. Plant Leaves on the Mitosis of Root Tips of Allium Cepa
Article Sidebar
-
Allium Cepa, Chromosomal Aberrations, Cytotoxic, Dodonaea Viscosa L, Extract
Abstract
This study aims to evaluate the genotoxicity of acetone extract of Dodonaea viscosa L. plant leaves on the mitosis in root tips of Allium cepa using three concentrations (6, 0.6, and 0.06) mg/ml, for 24 hrs of treatment. depending on some cytogenetic analysis such as mitotic index, phase index and chromosomal aberration. The results showed that treatment with all extract concentrations resulted in a decrease in the percentage of mitotic index , this percentage decreased to less than 50% with the treatment of 6 mg/ml compared to the control group , also the results showed that the extract treatment resulted in different chromosomal aberrations especially when treated with the concentration of 0.6 mg/ml which showed a significant increase at P <0.05 in the mean values of the total number of chromosomal aberrations in all stages of mitosis compared to the control group and concentrations of (6, 0.06) mg/ml. The chromosomal aberrations observed in this study were represented in fragments, stickiness chromosomes, lagging chromosomes, chromosomal bridges, breaks, granulated prophase, disturbed chromosomes and clumped chromosomes. We concluded from this study that Dodonaea viscosa L. , which is used in many medical fields, has a genotoxic effect, as the results of this study showed that the treatment with acetone extract of this plant led to the inhibition of mitosis in the root tips of Alium cepa and induced of many chromosomal aberrations at all stages of mitosis.
Full text article
References
1- أبوزيد، الشحات نصر (2006) فسيولوجيا وكيمياء القلويدات في النباتات الطبية وأهميتها الدوائية والعلاجية / الطبعة الأولي / دار الكتب العلمية للنشر والتوزيع القاهرة مصر.
2- العبيدي، هاشم كاظم (2016) زيادة بعض المركبات الفلافونيه الطبية لنبات الدودونيا باستخدام نترات الفضة النانوية خارج الجسم المي المجلة العراقية للعلوم، المجد 57— العدد 1ب- الصفحات 338—343 / بغداد- العراق.
3- السعدي، رشا كريم محمد (2013) التأثير الوراثي الخلوي للمستخلص المائي الخام لجذور نبات الفجل L. sativus Raphanus على خلايا القمم النامية لجدور البصل .L cepa AIIium ، مجلة جامعة النهرين، المجلد 16 - العدد 1 - الصفحات 12—19.
4- عفيفى ، فتحي عيد العزيز، عطى، محمود السيد (2003) المستخلصات النباتية والفاعلية البيولوجية / الطبعة الأولى / مكتبة الثقافة الدينية - مصر.
5- عريش، فاطمة سليمان، الجنقة، أحمد علي (2015) تقييم السمية الوراثية للمركبات الجليكوسيدية المستخلصة من أوراق وثمار نيات العشار Ca1otropis procera على الانقسام الميتوزي في القمم النامية لجذور نبات البصل Cepa A1lium ، مجلة جامعة سبها (العلوم البحثية والتطبيقية) 14، الصفحات 14—27.
[6]-Al-Ahmadi, M. S. ( 2013) Effects of organic insecticides, Kingbo and Azdar 10 EC, on mitotic chromosomes in root tip cells of Allium cepa. International Journal of Genetics and Molecular Biology. 5(5): 64-70.
[7]-Alagarsamy V. (2007) Antiinflammatory activity of Dodonaea viscosa linn. leaf extracts. Indian Drugs. 44(7) : 559-560.
[8]-Anderson, D. and Conning, D. M. (1993) Experimentaltoxicology the basic issues. 2nd edition, the Royal society of chemistry. pp: 254 –255.
[9]-Antonise–Wiez, D. (1990) Analysis of the cell cycle in the root meristem of Allium cepa under the influence of ledakrin. Hiostochem. Cytobiol. 26:76-96.
[10]-Cermak, R. and Wolffram, S. ( 2006 ) The potential of flavonoids to influence drug metabolism and pharmakinetics by local gastrointestinal mechanisms. Curr Drug Metab. 7(7):44-729.
[11]-EL-ghamery, A. A. (2004) Practical cytology. University of Al Azhar. Egypt.
[12]-EL-Gharmery, A.; EL-Nahas, A. and Mansour, M. (2000) The action of atrazine herbicides as inhibitor of cell division on chromosomes and nucleic acide contents in root meristems of Allium cepa and Vicia faba. Cytologia. 55: 209-215.
[13]-Fiskesjö, G. (1989) Allium test Protocol no 8, Invit tox on line.
[14]-Getie, M.; Mariam, T. G.; Rietz, R.; Hohne, C.; Huschka, C.; Schmidtke, M. A. and Neubert, R. H. (2003) Evaluation of anti-microbial and anti –inflammatory activities of the medicinal plants. Dodonaea viscosa, Rumex nervosus and Rumex abyssinicus. Fitoterapia.74(1-2) :139-143.
[15]-Grant, W.f. and Zura, K. D. (1982) Plants as sensitive in situ detectors of atmospheric mutagens. In: Heddle, J. A. (Ed.) Mutagenicity: New Horizons in Genetic Toxicology, Academic Press, New York, pp. 407-434.
[16]-Grant, W. f., Zinoveva-Stahevitch, A. E. and Zura, k. D.(1981) Plant genetic test systems for the detection of chemical mutagens. In: Stich, H. F. And San, R. H. C. (Eds) Short-Term Tests for Chemical Carcinogens , Springer -Verlag , New York, Heidelberg, Brlin, pp: 200-216.
[17]-Grover, I. S. and Kaur, S. (1999) Genotoxicity of wastewater samples from sewage and industrial effluent detected by the Allium cepa root anaphase aberration and micronucleus assays. Mutation Research, Amsterdam, v. 426, n. 2, p. 183-188.
[18]-Khuram, M.; Khan, A.; Hamed, A. and Abbas, N. (2009) Antibacterial.
[19]-Activity of Dodonaea viscosa using Contact Bioautography Technique. Molecules. 14 : 1332-1341.
[20]-Lawal, D. and Yunusa, L. (2013) Dodonea Viscosa Linn: Its medicinal, phytochemical properties. International journal of innovation and Applied studies. (2) 4 : 477-483.
[21]-Malkhan, S. G.; Shahid, M. K. and Kangabam, S. S. ( 2012) Efficacy of plant extractsin plant disease management. Agric. Sci.3(3) : 425-433.
[22]-Muthukumran, P. Hazeena V. and Kalaiarasan, P. (2011) Anti-Diabetic activity of Dodonaea viscosa (L) Leaf Extracts, International Journal of PharmTech Research CODEN (USA): 3(1) : 136-139.
[23]-Sandhya, R.; Rao –Pippalla, S. and Mohan, K. ( 2009) An overview of Dodonea Viscosa Linn. J.P.R.H.C. 1: 97-112.
[24]-Saggoo, M. I. S. Kumari , S. and Bindu(1991).Cytological effects of India medicinal plants I. Mitotic effects of leaf homogenate of tylophoraindical on Allium cepa.Cytologia, 56:33-637.
[25]-Sakthivadivel, M. and Daniel, T. (2008) Evaluation of certain insecticidal plants for the control of vector mosquitoes viz. Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti.Appl.Entomol. Zool. 43(1) pp: 57-63.
[26]-Shugeng, C.; Peggy, B.; Martin, C.; Richard, R.; Jeremi, R.; Etienne, R.;Vincent, R.; Karen T.;| Yongchun, S.; Edward, S.; and David, K.; (2009) Antiproliferative Triterpenoid Saponins of Dodonaea Wiscosa from the Madagascar Dry Forest1. Journal of Natural Products, 2009, Vol. 72, No. 9, p.1705-1706.
[27]-Veerapu, V. P.; Prabhakar, K.R.; Thippeswamy, B.S.; Srinivasan, K. and Unnikrishnan, M.K. (2010) Antidibetic effect of Dodonaea Viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study Indian Journal of Experimental Biology. 48: 800-810.
[28]-West, J. G. (1984) A taxonomic revision of Dodonea (Sapindaceae) in Australia, Brunonia .7:1-194.
Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
In a brief statement, the rights relate to the publication and distribution of research published in the journal of the University of Sebha where authors who have published their articles in the journal of the university of Sebha should how they can use or distribute their articles. They reserve all their rights to the published works, such as (but not limited to) the following rights:
- Copyright and other property rights related to the article, such as patent rights.
- Research published in the journal of the University of Sebha and used in its future works, including lectures and books, the right to reproduce articles for their own purposes, and the right to self-archive their articles.
- The right to enter a separate article, or for a non-exclusive distribution of their article with an acknowledgment of its initial publication in the journal of Sebha University.
Privacy Statement The names and e-mail addresses entered on the Sabha University Journal site will be used for the aforementioned purposes only and for which they were used.