Selection of Salinity Tolerant Rhizobial Isolates from Wild and Cultivated Legumes in Arid Region of Libya (Fezzan)
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Isolate, Legumes Plant, Fezzan, Sodium Chloride, Medicago Sativa, Symbiosis
Abstract
Eighty-four isolates were isolated from different legumes (Pisum sativa, Vicia faba, Cicer arietinum, Medicago sativa, Trigonella foenumgraecum, Lotus halophils, Astragalus fructicus, Hippocarpis, Viciae rvilla, V. narbonensis, Medicago polymorph and M. littoralis) Planted or wild in different locations of the dry zone of Libya (Fezzan) These isolates were tested for their ability to tolerate the salts of sodium, potassium and magnesium chloride on yeast extract and menthol; some rhizobia isolates of the fenugreek (Trigonella foenumgraecum) It was tested on alfalfa plant (Medicago sativa) This is to determine the level of sodium chloride salt, which allows the formation of Symbiosis and nitrogen fixation. Results of the salinity test on the steel medium were subjected to numerical analysis (numerical taxonomy), where three groups were formed. Three groups were formed. Most of the isolates of the first group were resistant to all tested salts. They grew at a salinity level of 8.5 to 9.5%. The isolates of the second group were sensitive to sodium and magnesium chloride salts and less resistant to potassium salt. Group III isolates were different in their response to salts tested, the results of the test of the growth of some saline resistant isolates in the liquid medium were similar to their growth on the middle of the agar, where the concentration allowed growing between 5-8% of sodium chloride, the growth-inhibiting focus was between 6-9%, effect of sodium chloride salt on the symbiosis between alfalfa plant and some isolates (T3 and T5) from the fenugreek showed that the maximum concentration allowed for the formation of Symbiosis was between 0.8 and 1% of chloride Sodium, the higher the concentration of salt, the smaller the number of nodes formed, and the 1.5% level was a disincentive to the Symbiosis process.
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References
[1]- FAO 2006. World agriculture: Towards 2030 / 20510. Prospect for Food, nutrition agriculture and major commodity groups. Global perspective studies unit Food and Agriculture Organization of the United Nations, Rome, Italy.
[2]- Ashraf, M. and Harris, P. J. C. 2005. A biotic stresses plant resistance Through Breeding and Molecular Approaches Haworth press, New York.
[3]- FAO 2008. Land and plant nutrition managem-ent service. htpp:// www.fao.org/agl/agll/spush.
[4]- Carter, O. L. 1975. Problems of salinity in agriculture. P. 25-39. In A.Poljakoff-Mayber and J Gale (ed.) Plants in saline environments. Springer-Verlag, New York.
[5]- Munns, R. 2002b. Salinity growth and phytohormones. In Lauchli A, Luttge U (eds) Salinity: environment-plants-molecules. Kluwer, The Netherlands, pp 271-290.
[6]- Brouwer, C., Goffeau, A. and Heibloem, M. 1985. Irrigation water management: training manual No. 1-Introduction to irrigation. Food and Agriculture Organization of United Nations, Rome, Italy.
[7]- إير اهيم، عيد الباسط عودة 2011، الإجهاد الملحى.
[8]- Young, J. M. 2003. The genus name Ensifer Casida 1982 takes priority over Sinorhi-zobiumChen et al. 1988, and Sinorhizobium morelense Wang et al. 2002 is a later Syno-nym of n EnsiferadhaerensCasida 1982. Is the combination Sinorhizobium adhaerens(Casida1982) Willems et al. 2003 legitimate? Request for an opinion Int J Syst Evol Microbiol 53: 2107-2110.
[9]- Weir, B. S. 2006. The current taxonomy of rhizobia, New Zealand rhizobia website, http:// www. rhizobia. Co. nz/ taxonomy/ rhizobia. html Regularly updated.
[10]- Young, J. P. W. 1992. Phylogenetic classification of nitrogen-fixing organisms. In Biological Nitrogen Fixation ( Stacey, G.et al., eds), Pp 43-86, Chapman and Hall.
[11]- Zahran, H. H. 1991. Conditions for successful Rhizobium-legume symbiosis in saline environments. Biol Fertil Soil 12: 73-80.
[12]- Abdelnaby, M., Elnesairy, N. N. B., Mohamed, S. H., and Alkhayali, Y. A. A. 2015. S-ymbiotic and Phenotypic Characteristics of Rhizobia Nodulating Cowpea (Vigna unguiculataL. Walp) Grown in Arid Region of Libya (Fezzan). J Enviro Sci and Engine 227-239.
[13]- Zahran, H. H. 1999. Rhizobium-legume symbiosis and nitrogen fixation under severe condition and in arid climate. Microbiol Mol Biol Rew 63:968-989.
[14]- ادهام، العسافي على؛ حمزة، ياس خضير؛ حمودي، دنيا ثامر 2010. تثير الملوحة في نمو بعض Bradyrhizobium japonicum، مجلة الانيار للعلوم الزراعية المجلد :8 العدد (4)- كلية الزراعة جامعة الأنبار/ العراق.
[15]- Abdelmoumen, H. and El Idrissi, M. M. 2009. Germination, growth and nodulation of Trigonellafoenumgraecum(Fenu Greek) under salt stress. Afr J Bio. Vol 11: 2489 -2496.
[16]- Vincent, J. M. 1970. A manual for the practical study of root-nodule bacteria. In: International Biological program me, Handbook no. 15. Oxford, Blackwell Scientific Publicat-ion Ltd, pp. 73-97.
[17]- Aneja, K. 2003. Experiments in Microbiology plant pathology and Biotechnology, 4th Ed, New Age. 4International Publishers, New Delhi, India.
[18]- Sneath, P. H. A. and Sokal, R. B. 1973. Numerical Taxonomy: the principles and practices of Numerical classification,. Freeman. W. H. and Co., San Francisco, USA.
[19]- Elsheikh, E. A. E. 1998. Effects of salt on rhizobia and Bradyrhizobia. Ann. App. Biol, 132: 507-524.
[20]- Jordan, D. C. 1984. Family III. Rhizobaceae Conn 1938. In Bergy̕s manual of syste-matic bacteriology. Vol. 1. Edited by N. R. Krieg and J. G.
[21]- Holt. Williams and Wilkins, Baltimore, Md. pp. 234-244.
[22]- Abdel-salam, M.S., Ibrahim, S. A., .Abd EL –Halim, M . M., Badawy, F. M. and Aba, S . E . M. 2010. Phenotypic characterization of indigenous Egyptian Rhizobial strains for abiotic stresses performance. J American Sci . 6 (9).
[23]- Mohamed, S. H., Smouni, A., Neyra, M., Kharchaf, D. and Filali-Maltouf, A. 2000. Phenotypic characteristics of root nodulating bacteria isolated from Acaciaspp. growing in Libya. Plant and Soil 224: 171-183.
[24]- Khalifa, M. O. A., Babiker, N. N. and Mohamed, S. H. 2014. Physiological characte-ristics of rhizobia isolated from Retama raetam(Forsk) and Lupinus various(L.) indigenous to Libyan desert. J Enviro Sci Engine 246-255.
[25]- El Sadi, S. M. A. 2004. Characteristics of root-nodulation bacteria isolated from Acacia karroo in Fezzan region of Libya. MSc. Thesis, Faculty of Sciencs, Sebha University, Sebha Libya.
[26]- Maatallah, J., Berraho, E., J. Sanjuan, and Lluch, C. 2002. Phenotypic characterizati-on of rhizobia isolated from chickpea (Cicer arietinum) growing in Moroccan soils. Agronomie, 22: 321-329.
[27]- Craig, G. F., Atkins, C. A. and Bell, D. T. 1991. Effect of salinity on growth of Rhizo-biumand their infectivity and effectiveness on two species of Acacia. Plant soil 133: 253 –262.
[28]- Shereen, A., Ansari, R., Naqvi, S. S. M. and Soomro, A. Q. 1998. Effect of salinity on Rhizobiumspecies nodulation and growth of Soybean (Glycine maxL.). University of sind, Pakistan, 30 (1): 75-81.
[29]- EL-Mokadem, M. T., Helemish, F. A., Abdel-Wahab, S. M. and Abou-El –Nour, M. M. 1991. Salt response of clover and alfalfa inoculated with salt tolerant strains of Rhizobi-um. Ani Shams Sci Bull 28B: 441-468.
[30]- Jida, M. and Assefa, F. 2011. Phenotypic and plant growth promoting Characteristics of Rhizobiumleguminosarumbv. Vicia from Lentil growing areas of Ethiopia. African Journal of microbiology Research vol. 5(24): pp. 4133-4142, 30 October, ISSN 1996-0808@ 2011 Academic Journals Addis Ababa University, Ethiopia.
[31]- Dudeja, S. S., Khurana, A. L. and Kundu, B. S. 1981. Effect of Rhizobiumand phos-phomicroorganisms on yield and nutrient uptake in chickpea, Curr. Sci. 50: 503-505.
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