Positive Effects of Nigella sativa Against CCl4-induced Hepatic Toxicity in male rats : A Biochemical and Histological Study
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Hepatic Toxicity, CCl4 , Nigella sativa, Blood changes, histological changes.
Abstract
This investigation was aimed to study the effect of acute carbon tetrachloride (CCL4) toxicity and to evaluate the protective role of diet supplementation of black seeds powder (NSS) at a level of 20 % on amelioration of the reversal effect of carbon tetrachloride-induced hepatotoxicity on biochemical and histological levels in rats model. Twenty eight adult rats (160 – 200 g body weight ) were allocated into four equal groups: group (1) control rats given sunflower oil only, group (2) were injected intraperitonelly (IP) with CCL4 (0.2 ml/100 gm body weight.) mixed with ( 1:1 V/V ) of sunflower oil for three consecutive days , Group 3 (pretreatment experiment-NSS ): feed with standard diet plus 20% of Nigella sativa and CCl4 IP on Days 14, 15, and 16 of the treatment period, while Group 4 (post-treatment experiment-NSS ) treated with IP CCl4 on the first three consecutive days of the treatment period plus 20% of NSS for 14 other days and continued with standard diet 24 hours after the last treatment , the rats were euthanized; blood samples were collected for biochemical analysis and liver sections were prepared for histological examination. The present data demonstrated that, injection of CCL4 to rats increased significantly ( p<0.05( triglycerides, total cholesterol, LDL, HDL and total lipid as compared with normal serum control rats. While the treatment with Nigella sativa seed powder decreases significantly high values of lipids in rats treated with CCL4. Histological studies of liver also showed protective effect of NSS. We have observed thickening of blood vessels and micro-vesicular fatty changes around the portal triad in CCl4-treated rat liver. Treatment with NSS. showed only mild sinusoidal dilatation and mild portal inflammation. From the previous results, it may be concluded that feeding rats with grinded seeds of Nigella sativa as part of food supplement to CCL4 administered rats may have protective and safe effect against hepatotoxicity which may be due to its antioxidant activity.
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