Cultured of Leaves and Stems of Tragnum Nudatum In Vitro
Article Sidebar
-
Tragnum, Leaves, Stems, Callus, In Vitro
Abstract
The leaves and stems of Tragnum nudatum were cultured on MS medium supplemented with different concentrations of 2,4-D, BA, and kin. When leaves were inoculated on MS+Kin (1 mg/l), high amounts of friable callus were obtained after 10 weeks. No responses were noticed on other treatments after 12 weeks. Those calluses were subcultured on the same medium; they showed increases in growth when compared with other treatments. In case of cultured stems, no responses were recorded in all combinations even after 12 weeks of culture.
Full text article
References
1- أمحمد، أبويكر ابراهيم، الخيالي، يونس (2005) تطبيقات تقنية زراعة الانسجة النباتية على نبات الرسو الصحراوي Comosum CaIIigonum المؤتمر الوطني الثالث للهنيات الحيوبة 145-138.
2- العابد إبراهيم (2009) دراسة الفعالية المضادة للبكتيريا والمضادة للأكسدة لمستخلص القلويدات الخام لنبات الضمران، جامعة قاصدي مرياح ورقلة. رسالة ماجستير.
[3]Jafri.S.M.H.,El-Gadi.A. 1983. Flora. Of Libya Al-faateh University, faculty of Science Tripoli.106-2-4.
[4]Mahmoud O.,Kosr M (2013). Regeneration and Histological of plants dervied from leaf explants in vitro culture of Strawberry. International jounral of Agriculture and Crop Sciences.5(9):943-950.
[5]USDA, Information Resources (2010) .GermplasmResources Network -Grin-National Genetic Program. National Germplasm Resources Laboratory Beltsville,Maryland.
[6]T.and Skoog, F.1962.A revised medium for rapid, Murashige Growth and bioassays with tobacco tissue plantarum15:473-479.
[7]Yadav K,Singh N(2011). in vitro propagation and biochemical analysis field established wood Apple (Agle marmelon .Anaele, Universitatii din Oradea-fasciulua.(18):23-28.
[8]Rajore S., Batra A.2007.An alternative source for regenerable organogenic callus induction in L.indian journal of Jatropha curcas biotechnology.6(5):45-548.
[9]Nehra N S., Chibbar R N., Karth K K.(1990).Genetic transformation of Strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system.plant cell reports.9(6):293-298.
[10]Kakaria L., Rama C., Botlagunta M.2014. Somatic embryogenesis and plant regeneration from leaf explants of Rumex vesicarius .African journal of bioitchnology.
[11]George E.,hall M.,Jan de klerk G.2008.Plant tissue culture procedure-background.in Plant propagation by tissue culture. 3ed,springer, Dordrecht. Netherland.1-28.
[12]Agastian P.,Willimas I., Ignacimuthu S.(2006).In vitro propagation of Justicia gendrussa Burm.f.-A medicinal plant.Indian biotechnology.5:24-248.
[13]Saad A., Abdulla K.,Emhemed F.,(2016).Anatomy study of seeds of Calligonum comosum in vitro. World Academy Science, Engineering and Technology.3.
[14]Saad A., Abdaslam A.2017.In vitro culture of stem node segments of Maerua crassifolia.World Academy of Science, Engineering and Technology.4:4-10.
Authors
Copyright (c) 2019 Journal of Pure & Applied Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
In a brief statement, the rights relate to the publication and distribution of research published in the journal of the University of Sebha where authors who have published their articles in the journal of the university of Sebha should how they can use or distribute their articles. They reserve all their rights to the published works, such as (but not limited to) the following rights:
- Copyright and other property rights related to the article, such as patent rights.
- Research published in the journal of the University of Sebha and used in its future works, including lectures and books, the right to reproduce articles for their own purposes, and the right to self-archive their articles.
- The right to enter a separate article, or for a non-exclusive distribution of their article with an acknowledgment of its initial publication in the journal of Sebha University.
Privacy Statement The names and e-mail addresses entered on the Sabha University Journal site will be used for the aforementioned purposes only and for which they were used.