Cultured of leaves and stems of Tragnum nudatum in vitro
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Tragnum, leaves, Stems, callus, in vitro.
Abstract
The leaves and stems of Tragnum nudatum were cultured on MS medium supplemented with different concentrations of 2,4-D,BA,kin .When leaves were inoculated on MS+Kin (1mg/l) high amounts of friable callus were obtained after 10 weeks. No responses were noticed on other treatments after 12 weeks. Those calluses were subcultured on same medium, they showed increases in growth when compared with other treatments. In case of cultured stems , no responses were recorded in all combinations even after 12 weeks of culture.
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