Parasitic Contamination of raw vegetables sampled from different farm locations in Brack Al-Shati, Libya
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Brack Al-Shati, Contamination, Intestinal parasites, Libya, Vegetables
Abstract
Background: Fresh Vegetables provide important nutritional value for humans; however, consumption of raw vegetables can be a major route for transmission of food-borne parasitic diseases. Hence, this study aimed to investigate the parasitic contamination of commonly consumed local vegetables in Brack Al-Shati, Libya. Methods: A total of 200 samples of fresh vegetables were randomly collected from vegetable farms in Brack Al-Shati, Libya. The vegetables included; green onion, lettuce, rocket, coriander, parsley, chard, mint, cucumber, carrot, tomato, and turnip. Samples were washed in saline, and the resulting washing solution was filtered and centrifuged to concentrate the parasitic stages. The sediments were examined by iodine and modified Ziehl-Neelsen stained smears. Results: The percentage of contamination was 33.0% (66/200), with predominance of nematode larvae (37.9 %), eggs of Ascaris (22.7%), cysts of Entamoeba spp. (18.2%), oocysts of Cryptosporidium spp. (16.7%), cysts of Giardia lamblia (3.0%) and eggs of Hymenolepis nana (1.5%). The highest number of contaminated samples was detected in rocket (70.1%) while the least number of contaminated samples was detected in green onion (6.7%). No parasite was detected in tomato samples. Conclusion: The study highlighted the importance of vegetables as the potential source for transmission of intestinal parasites to humans. Vegetables contaminated with the pathogenic parasites may pose health risk to the consumers if consumed without proper cleaning and or cooking.
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References
[1] Slavin, J. L., & Lloyd, B. (2012). Health benefits of fruits and vegetables. Advances in Nutrition, 3(4), 506–516. mansour,+036Parasitic+Contamina…
[2] Winston, C., & Beck, L. (1999). Phytochemicals: health protective effects. Canadian Journal of Dietetic Practice and Research, 60(2), 78.
[3] Kniel, K. E., Lindsay, D. S., Sumner, S. S., Hackney, C. R., Pierson, M. D., & Dubey, J. (2002). Examination of attachment and survival of Toxoplasma gondii oocysts on raspberries and blueberries. Journal of Parasitology, 88(4), 790–793.
[4] Amoah, P., Drechsel, P., Abaidoo, R., & Klutse, A. (2007). Effectiveness of common and improved sanitary washing methods in selected cities of West Africa for the reduction of coliform bacteria and helminth eggs on vegetables. Tropical Medicine & International Health, 12, 40–50.
[5] Beuchat, L. R. (2002). Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes and Infection, 4(4), 413–423.
[6] Slifko, T. R., Smith, H. V., & Rose, J. B. (2000). Emerging parasite zoonoses associated with water and food. International Journal for Parasitology, 30(12–13), 1379–1393.
[7] Damen, J., Banwat, E., Egah, D., & Allanana, J. (2007). Parasitic contamination of vegetables in Jos, Nigeria. Annals of African Medicine, 6(3), 115–118.
[8] Gharavi, M., Jahani, M., & Rokni, M. (2002). Parasitic contamination of vegetables from farms and markets in Tehran. Iranian Journal of Public Health, 31(3–4), 83–86. mansour,+036Parasitic+Contamina…
[9] Alhabbal, A. T. (2015). The prevalence of parasitic contamination on common cold vegetables in Alqalamoun Region. International Journal of Pharmaceutical Sciences Review and Research, 30(1), 94–97.
[10] Al-Megrm, W. I. (2010). Prevalence of intestinal parasites in leafy vegetables in Riyadh, Saudi Arabia. International Journal of Tropical Medicine, 5(2), 20–23.
[11] Fagbenro, M., Mogaji, H., Oluwole, A., Adeniran, A., Alabi, O., & Ekpo, U. (2016). Prevalence of parasites found on vegetables and perception of retailers and consumers about contamination in Abeokuta Area of Ogun State, Nigeria. 2(1).
Editorial note: the journal/source title is not clearly supplied in the manuscript.
[12] Garedaghi, Y., Farhang, H., & Pooryagoobi, S. (2011). Parasitic contamination of fresh vegetables consumed in Tabriz, Iran. Research Journal of Biological Sciences, 6(10), 518–522.
[13] Said, D. (2012). Detection of parasites in commonly consumed raw vegetables. Alexandria Journal of Medicine, 48, 345–352.
[14] Mohamed, M. A., Siddig, E. E., Elaagip, A. H., Edris, A. M. M., & Nasr, A. A. (2016). Parasitic contamination of fresh vegetables sold at central markets in Khartoum State, Sudan. Annals of Clinical Microbiology and Antimicrobials, 15(17). DOI: 10.1186/s12941-016-0133-5.
[15] Daryani, A., Ettehad, G., Sharif, M., Ghorbani, L., & Ziaei, H. Prevalence of intestinal parasites in vegetables consumed in Ardabil, Iran. Food Control, 19(8), 790–794.
[16] Abougrain, A. K., Nahaisi, M. H., Madi, N. S., Saied, M. M., & Ghenghesh, K. S. Parasitological contamination in salad vegetables in Tripoli-Libya. Food Control, 21(5), 760–762.
[17] Luz, J., Barbosa, M., de Carvalho, A., Resende, S., Dias, J., & Martins, H. (2017). Contamination by intestinal parasites in vegetables marketed in an area of Jequitinhonha Valley, Minas Gerais, Brazil. Revista de Nutrição – Brazilian Journal of Nutrition, 30(1), 127–136.
[18] Mahdi, W. A., Dawod, Y. T., & Al-Qadhi, B. N. (2013). Survey study of intestinal parasites on fresh vegetables collected from some Baghdad sales and its role in human infection. Baghdad Journal of Science, 10(1), 32–40.
[19] Bekele, F., Tefera, T., Biresaw, G., & Yohannes, T. (2017). Parasitic contamination of raw vegetables and fruits collected from selected local markets in Arba Minch town, Southern Ethiopia. Infectious Diseases of Poverty, 6, 19. DOI: 10.1186/s40249-016-0226-6.
[20] Hajjami, H., Ennaji, M. M., Amdiouni, H., Fouad, S., & Cohen, N. (2013). Parasistic contamination on fresh vegetable consumed in Casablanca City (Morocco) and risk for consumer. International Journal of Science and Technology, 2(7), 543–549. mansour,+036Parasitic+Contamina…
[21] Kozan, E., Gonenc, B., Sarimehmetoglu, O., & Aycicek, H. (2005). Prevalence of helminth eggs on raw vegetables used for salads. Food Control, 16(3), 239–242.
[22] Dorris, M., Viney, E., & Blaxter, M. (2002). Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. International Journal for Parasitology, 32(12), 1507–1517.
[23] Schär, F., Trostdorf, U., Giardina, F., Khieu, V., Muth, S., Marti, H., Vounatsou, P., & Odermatt, P. (2013). Strongyloides stercoralis: global distribution and risk factors. PLoS Neglected Tropical Diseases, 7(7), e2288.
[24] Al-Binali, A. M., Bello, C. S., El-Shewy, K., & Abdulla, S. E. (2006). The prevalence of parasites in commonly used leafy vegetables in South Western Saudi Arabia. Saudi Medical Journal, 27, 613–616.
[25] Fallah, A. A., Pirali-Kheirabadi, K., Shirvani, F., & Saei-Dehkordi, S. S. (2012). Prevalence of parasitic contamination in vegetables used for raw consumption in Shahrekord, Iran: Influence of season and washing procedure. Food Control, 25(2), 617–620.
[26] Adejumoke, A., & Morenikeji, O. (2015). Prevalence of intestinal parasites in vegetables sold in major markets in Ibadan city, south-west Nigeria. Global Journal of Pure and Applied Sciences, 21(1), 7–12.
[27] Roberts, A. (2009). Ascariasis: Introduction and epidemiology and transmission. In Medical Parasitology. Austin, Texas: Landes Bioscience, pp. 14–20.
[28] Ulukanligil, M., Seyrek, A., Aslan, G., Ozbilge, H., & Atay, S. (2001). Environmental pollution with soil-transmitted helminths in Sanliurfa, Turkey. Memórias do Instituto Oswaldo Cruz, 96, 903–909.
[29] Macarisin, D., Bauchan, G., & Fayer, R. (2010). Spinacia oleracea L. leaf stomata harboring Cryptosporidium parvum oocysts: a potential threat to food safety. Applied and Environmental Microbiology, 76, 555–559.
[30] Silva, C., Andrade, S., & Stamford, T. (2005). Occurrence of Cryptosporidium spp. and other parasites in vegetables consumed in natura, Recife, Brazil. Ciência & Saúde Coletiva, 10, 63–69.
[31] Eraky, M. A., Rashed, S., Nasr, M. E.-S., El-Hamshary, A. M. S., & El-Ghannam, A. S. (2014). Parasitic contamination of commonly consumed fresh leafy vegetables in Benha, Egypt. Journal of Parasitology Research, 2014, Article 613960. DOI: 10.1155/2014/613960. mansour,+036Parasitic+Contamina…
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