Virulence Characteristics of Multiple Drug Resistant Uropathogenic Escherichia Coli from Patients in Some Clinics of Sebha
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Multi Drug Resistance, Urinary Tract Infections, Uropathogenic Escherichia Coli, Virulence Factors
Abstract
Urinary tract infections are the most common health problems in many countries of the world. Although E. coli is normal intestinal flora, it is considered the main prominent opportunistic active uropathogen because of its pathogenicity, which refers to its different virulence factors like hemolysin, biofilm, enterotoxins, and others. In this study, 18 negative bacterial species were isolated from 30 urine samples of outpatients of some clinics in Sebha City, where Escherichia coli was the most common bacterial species in the patients of both sexes (19.15%). Virulent strains were identified based on their resistance to antibiotics, hemolysis, and cell surface hydrophobicity as well as the formation of biofilm. The sensitivity test of Uropathogenic E. coli (UPEC) strains showed that all isolates were multidrug resistant, as 100% of the strains were resistant to antibiotics like oxacillin, penicillin, ampicillin, and amoxicillin, while only (13%) of the strains were resistant to nitrofurantoin, ciprofloxacin, and imipenem. The results showed that all isolates had not the ability to produce hemolysin, but they differed in their ability to form biofilm, where (33.3%) of the strains formed a thick biofilm, while) 40%( formed moderate biofilm and only (26.7%) of the strains formed weak biofilm. Additionally, we found that (66.6%) of the strains carried hydrophobicity markers, while (33.3%) of the UPEC strains did not have those markers. Occurrence of virulence factors in UPEC strains confirms the association of UPEC with urinary pathogenicity and their resistance to antibiotics.
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References
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