Screening the Ability of Several Microbial Isolates for Production of Some Industrial Enzymes
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Pectinase, Cellulase, A.Niger, B.Mycoide, E. Melioti
Abstract
Microbes release more stable enzymes, which can be obtained at the lowest coast. Species of fungi and bacteria were isolated from different sources, including Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium solani, Pseudomonas aeruginosa, Bacillus mycoides, and Bacillus cereus, as well as two isolates of Rhizobia, BUS26 and RMI4 in addition to the Ensifer meliloti as a reference strain of rhizobia. These microbial species were used to determine their ability to produce some industrial enzymes after culturing them on Czapek dox agar by replacing the carbon source with pectin, chitin, or cellulose. Then the average diameter of the colony was measured and the percentage of the enzymes production was calculated. The results showed that all the fungus utilized in this study produced the enzymes in different proportions. A. niger isolated from air and soil was able to grow and use orange peel as a single source of carbon to produce pectinase with the highest percentage of 80.77% and 71.72% respectively. As well as the highest yield of cellulase was produced by A. niger with a rate of 81.15%, while the rest of fungi differed in their ability to produce the enzymes between the medium and low amount. F. solani had the lowest ability to produce pectinase and chitinase by rates of 19.21% and 17.03% respectively. The ability of bacterial isolates to produce enzymes was also varied as B.mycoides and BUS26 gave the highest productivity of cellulase by 74.74% and 68.89% respectively. The productivity of pectinase was close as BUS26 isolate showed productivity rate of 50% following by P.aeruginosa with a ratio of 47.83% while B.cereus had a pectinase production with a rate of 34.66%.
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References
[1]-Bhardwaj, V. and Garg, N., (2010), Exploitation of micro-organism for isolation and screening of pectinase from environment. Globelics 2010 8th International Conference Making Innovation Work for Society: Linking, Leveraging and Learning. University of Malaya, Kuala Lumpur, Malaysia.
[2]-Cohen, G. N.,(2011),Microbiochemistry. 2ndEd.Springer.
[3]-Martins, E., Silva,D., Silva,R. and Gomes, E.,)2002(,Solid state production of thermostable pectinases from thermophilic Thermoascus aurantiacus. Process Biochem., 37,949-954.https://doi.org/10.1016/S0032-9592(01)00300-4
[4]-Silva, D., Martins, E., Silva, R. and Gomes. E.,(2002),Pectinase production by Penicillium viridicat-um RFC3 by solid state fermentation using agricul-tural wastes and agroindustrial by-products. Braz. J. Microbiol.,33,318-324.https://doi.org/10.1590/S1517-83822002000400008
[5]-White, S., Mclntyre,M.,Berry,D. and McNeil, B., (2002), The Autolysis of Industrial Filamentous Fungi. Critical Reviews in Biotechnology., 22(1),1-14.https://doi.org/10.1590/S1517-83822002000400008
[6]-Marin et al.,2004Martin, N., Souza, S., Silva, R. & Gomes, E. (2004). Pectinase production by fungal strains in solid –state fermentation using agro-industrial bioproduct. Brazilian Archives of Biology and Technollogy.47(5): 813-819.
[7]-Xia et al.,2011Xia, G., Jin, C., Zhou, J., Yang, S., Zhang, S., & Jin, C. (2001). A novel chitinase having a unique mode of action fromAspergillus fumigatusYJ-407. European Journal of Biochemistry, 268(14): 4079–4085.
[8]-Kotchon et al.,2006Kotchoni, S., Gachomo, E., Omafuvbe, B., & Shonukan, O. (2006). Purification andbiochemical characterization of Carboxymethyl Cellulose (CMCase) from a catabolite repression insensitive mutant of Bacillus pumilus. Int. J. Agri. Biol., 8(2): 286-292.
[9]-Tonozuka et al.,2014Tonozuka, T., Yoshida, M., & Takeuchi, M. (2014). Chapter 9 -Enzymes for Cellulosic Biomass Conversion. Research Approaches to Sustainable Biomass Systems,Pp 225–242.
[10]-Atlas,R., (2010), Handbook of Microbiology Media. 4thEd. Taylor and Francis Group LLC.
[11]-Leslie, J., and Summerell, B.,(2006),The FusariumLaboratoryManual. Blackwell Publishing. USA.
[12]-Watanabe, T.,(2010),Pictorial Atlas of Soil and Seed Fungi .3rdEd. Taylor and Francis Group LLC.
[13]-Campbell, C. K., Johnson, E. M., and Warnock, D. W.,(2013),Identification of pathogenic fungi .2ndEd. WILEY-BLACKWELL.
[14]-Abuzeid, M., Ahmadi. I., Khalifa, M., Almahdi, M., Mohamed, S., Sahl, N., and Ahmadi, F.,(2018),Virulence Characteristics of Multiple Drug Resistant Uropathogenic Escherichia colifrom patients in some clinics of Sebha. Journal of Pure & Applied Sciences., 17(1),175-182
[15]-Amin, M., Rakhisi, Z., and Ahmady, A. Z.,(2015),Isolation and Identification of BacillusSpecies from Soil and Evaluation of their antibiotic properties. Avicenna J Clin Microb Infec.,2(1),1-3.
[16]-AL-Humam, N. A.,(2016),Heat-Shock Technique for Isolation of Soil BacillusSpecies with Potential of Antibiotics Secretion in Saudi Arabia. British Microbiology Research Journal., 17(3),1-6.
[17]-Taskin, E., Eltem, R., Silva, E. S. D. and Souza, J.,(2008),Screening of Aspergillus strains isolated from vineyards for pectinase production. Journal of Food, Agriculture & Environment., 6,412-414.https://doi.org/10.1590/S1517-83822002000400008
[18]-Lennette, E. H., Spaulding, E. H. and Truant, J. P.,(1974),Manual of clinical microbiology. Am Soc for Microbiol Washington.
[19]-Abbott, W. S.,)1987(,A Method of Computing the Effectiveness of an Insecticide. Journal of The American Mosquito Control Association.,3)2(,302-303.
[20]-Okafor, U. A., Okochi, V. I., Chinedu, S. N., Ebuehi, O. T. and Onygeme-Okerenta, B. M.,)2010(,Pectinolytic activity of wild-type filamentous fungi fermented on agro-wastes. African Journal of Microbiology Research., 4(24),2729-2734
[21]-Hoster, F.; Schmitz, J. E. and Daniel, R. oster, F.; Schmitz, J. E. and Daniel, R.,)2006(,Enr-ichment of chitinolytic microorganisms: isolation and characterization of a Chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Appl MicrobiolBiotechnol., 66,434–442.10.1007/s00253-004-1664-9
[22]-Brzezinska, M. S., and Jankiewicz, U.,)2012(,Production of Antifungal Chitinase by Aspergillus niger LOCK 62 and Its Potential Rolein the Biological Control. Curr Microbiol., 65,666–672.https://link.springer.com/content/pdf/10.1007/s00284-012-0208-2.pdf
[23]-Karthik, N., Akanksha, K., Binod, P., and Pandey, A.,)2014(,Production, purification and properties of fungal chitinases—a review.Indian J Exp Biol., 52,1025-1035.10.1007/s00253-004-1664-9.
[24]-Dutt, D., and Kumar, A. )2014(,Optimization of Cellulase Production Uuder Solid-State Fermentation by Aspergillus flavus(AT-2) and Aspergillus niger(AT-3) and Its Impact on Stickies and Ink Particle Size of Sorted Office Paper. Cellulose Chem. Technol., 48(3-4),285-298.
[25]-Abdel-Fattah, G., Migahed, F., Soliman, H. and EL-Tanash, A., )2015(, Chitinolytic Activity of Filamentous Fungi: Isolation, Identification and Screening of Fungal Chitinase in Solid State Fermentation, Journal of Environmental Sciences, 44(1), 129-142.
[26]-Webster, J. and Weber, R.,)2007(,Introduction to Fungi. 3rdEd. Cambridge University Press.
[27]-Rathore, S. S., Mannivannan, A. and Narend-hirakannan R. T.,(2014),Screening of cellulose producing microorganisms from lake area containing water hyacinth forenzymatic hydrolysis of cellulose. Journal of Advanced Scientific Research., 5(3),23-30.
[28]-Oumer, O. J. and Abate, D. (2018), Screening and molecular Identification of pectinase producing microbes from coffee pulp. BioMed Research International., 20(8), 1-7.
[29]-Priya, C. S., Jagannathan, N. and Kalaichelv-an, P. T.,(2011),Production of chitinase of by Stre-ptomyces Hydro scopicus VMCH2 by optimization of cultural conditions. Int. J.25 Pharm. Biol. Sci., 2,210-219.
[30]-Jholapara, R., Mehta, R. S., Bhagwat, A.M., and Sawant, C. S.,)2013(,Exploring and optimizing the potential of chitinase production by Isolated Bacillusspp. International Journal of Pharmacy and Pharmaceutical Sciences., 5(4),412-418.
[31]-Nakamura, K., and Kappamura, K.,)1982(,Isolation and Identification of crystalline cellulose hydrolyzing bacterium and its enzymatic properties. J. Ferment. Technol., 60(4),343-348
[32]-Morales, V., Martienz-Molina, E., and Hubbell, D. (1984),Cellulase production by Rhizobium. Plant and Soil, 80(3),407-415.
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