Isolation and Identification of fungal genera growth on some Barley and rice samples retailed at Brack/Ashati supermarkets.
Article Sidebar
-
Rice, Wheat, Aflatoxin, Ochratoxin, Chlorine
Abstract
The Wheat, barley and rice are the mainly consumed cereals in Libya. This study aimed to isolate the fungal genera of these cereals with special focus on the mycotoxigenic fungi (Aspergillus) species. Samples type of rice (8 samples) and wheat (3 samples) were collected from the local market in Brack – Ashati, Libya Samples were found heavily contaminated with fungi either before or after surface sterilization with 2% chlorine solution, even though surface sterilization reduced the count but mycotoxigenic fungi still present. A total of 13 isolates were obtained and were identified through the morphological characters such as colony colour, mycelium diameter, spore size and colour and identified as Aspergillus niger, A. flavus, A. terreus, A. carbonarius, A. parasiticus, A. ochraceus, A. terreus, Mucor, Rhizopus and Cladosporium. Fungal isolates which suspected to produce mycotoxins were studied to investigate their ability to produce mycotoxins using ammonia solution (20%) and the results indicated aflatoxins and ochratoxin A production. The colour of the filter paper which hsa been previously immersed in ammonia solution indicate the presence of these mycotoxins. Presence of such fungal genera in foods can cause a severe problem to the health such as cancer and kidney failure.
Full text article
References
وهبة، ناهد محمد؛ النسر، نفين عبدالغني2010)). السموم الفطرية في الألبان ومنتجاتها والخطر والوقاية. مجلة أسيوط للدراسات البيئية، العدد الرابع والثلاثون، أسيوط، مصر.
السروي، ا . (2009 ). الثلوت البيئي الطبيعية المصدر – التأثيرات – المكافحة والتحكم. المكتبة الدار العالمية، القاهرة.
Andrews, S. (1986). Optimisation of conditions for the surface disinfection of sorghum and sultanas using solutions .In: A.D King ,J.I Pitt ,L .R. Beuchat and J. E. Cony (editors) ,Method for the Mycological Examination of food , Plenum Press ,New York,pp.28-32.
Pitt, J.I., Hocking, A.D., Samson, R.A. and King, A.D. (1992). Recommended methods for mycological examination of foods, 1992. In: R.A.Samson et al. (editors), Modem Methods in Food Mycology, Elsevier Science, Amsterdam, pp. 365-368.
Pitt,J.I.and Hocking,A.D.(1997).Fungi and food spoilage.Blackie Academic and professional.London. DOI: https://doi.org/10.1007/978-1-4615-6391-4
Perczak, A., Golinski, P., Marcin Bryla, M. and Waskiewicz, A. (2018). The efficiency of lactic acid bacteria against pathogenic fungi and mycotoxins. Arh Hig Rada Toksikol.69:32-45 DOI: https://doi.org/10.2478/aiht-2018-69-3051
Ciegter, A; Burmeister, H. R. and Vesonder, R. F. (2011). Poisonous fungi: Mycotoxin and mycotoxicosis in fungi pathogenic for human and animal. Part B. Pathogenicity and Detection: 1'' (D.H .Howard and L.F. Howard eds.). pp. 413-469.
Elzwai, J. I., Aidoo, K. E., Candlish, A. G. (2005). Mycroflora and occurrence of ochratoxin A in cereals of North African origin. Poster presented in the conference on Reducing Impact of Mycotoxins in tropical Agriculture with Emphasis on Health and Trade in Africa. Accra, Ghana, 13-16 September 2005.
Frisvad, J. C., Kristensen, A. B. and Filtenborg, O., Frisvad, J. C., Thrane, U. (1996). Moulds in food spoilage. International Journal of Food Microbiology, 33: 85-102 DOI: https://doi.org/10.1016/0168-1605(96)01153-1
Gilbert,j.(1984).Analysis of food contamination .Elsvier App.Sci.Pups.,London.
Rashid, M., Khalil, S., Ayub, N., Ahmed W. and Khan, A. (2008). Categorization of Aspergillus flavus and Aspergillus parasiticus isolates of stored Wheat grains in to aflatoxinogenics and non –aflatoxinogenics. Pakistan Journal of Botany,40:2177-219
Saito,M.andS.Machida,(1999). A rapid identification and A. parasiticus by ammonia vapor. Mycoscience,40:205-208. DOI: https://doi.org/10.1007/BF02464300
Samson, R., Hokstra, E. S., Frisvad, J. C. and Filteenborg, O. (2002). Methods for detection and isolation in food-born fungi Introduction to food fungi 6th Ed. Centraalbureau voor schimmel cultures ,Baarn pp76-77
Samson, R., Houbraken, J., Thrane,U., Frisvad, J., C. and Andersen, B. (2010). Food and Indoor Fungi CBS-KNAW Fungal Biodiversity centre, Utreach , The Netherlands.
Sauer, D. B., and Burroughd, R. (1986). Disinfection of seed surface with sodium hypochlorite. Phytopathology,76:745-749. DOI: https://doi.org/10.1094/Phyto-76-745
Verma, R.J. (2004). Aflatoxin cause DNA damage. Int. J. Hum. Genetic 4:231-236.1 DOI: https://doi.org/10.1080/09723757.2004.11885899
Zakrzewski, S. F (1991). Principle of environ mental toxicology .ACS professional reference book, Washington, DC,1
Vasanthi, S. and Bhat, R. V. (1998). Mycotoxins in food – occurrence ,health and economic significance and food control measures .Indian. J. Med. Res .,108:212-224.
Authors
Copyright (c) 2023 Journal of Pure & Applied Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
In a brief statement, the rights relate to the publication and distribution of research published in the journal of the University of Sebha where authors who have published their articles in the journal of the university of Sebha should how they can use or distribute their articles. They reserve all their rights to the published works, such as (but not limited to) the following rights:
- Copyright and other property rights related to the article, such as patent rights.
- Research published in the journal of the University of Sebha and used in its future works, including lectures and books, the right to reproduce articles for their own purposes, and the right to self-archive their articles.
- The right to enter a separate article, or for a non-exclusive distribution of their article with an acknowledgment of its initial publication in the journal of Sebha University.
Privacy Statement The names and e-mail addresses entered on the Sabha University Journal site will be used for the aforementioned purposes only and for which they were used.
