Evaluation of four commercial COVID-19 diagnostic RT-PCR kits used in Libya

Khadija M. Ahmad; Shamsi S Shamsi; Mohammed B Alsubbi; Ali A. Aldalouli; Hamad A Almahdi; Alkeelani M M Alkeelani; Ibrahim A. Mohamed

doi:10.51984/joms.v18i1.2600

Evaluation of four commercial COVID-19 diagnostic RT-PCR kits used in Libya

Authors

Khadija M. Ahmad Department of Microbiology, faculty of medicine, Sebha University,
Shamsi S Shamsi Department of Plant Science, Microbiology Division, Research Laboratory, Sebha University
Mohammed B Alsubbi General Department, Faculty of Nursing, Sebha University, Libya
Ali A. Aldalouli Department, PCR unit, clinical laboratory, Sebha Medical center.
Hamad A Almahdi Department, PCR unit, clinical laboratory, Sebha Medical center
Alkeelani M M Alkeelani Department, PCR unit, clinical laboratory, Sebha Medical center
Ibrahim A. Mohamed Department of Biochemistry, faculty of medicine, Sebha University.

DOI:

https://doi.org/10.51984/joms.v18i1.2600

Keywords:

Real-time PCR, COVID-19, molecular technique, respiratory infection, diagnostic kits

Abstract

Background: Real-time reverse transcriptase chain reaction is considered the most sensitive and specific assay for COVID-19 detection. This study was planned to compare and evaluate four commercially diagnostic kits. Material and methods: 92 Nasopharyngeal swabs collected from different symptomatic patients were studied in this project. The total nucleic acid (RNA) was extracted, and RT-PCR was done using four commercial diagnostic kits from different manufacturers, DANN and BGI (China), Hibrigen, and Bio-speedy (Turkey). Result: Although all 92 clinical samples were subjected to the same four diagnostic kits, the results revealed variations in the performance. The total number of positive cases was (42/92) for Da An Gene, BGI (36/92), Bio-speedy (31/92), and Hibrirgen (14/92).

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Author Biographies

Khadija M. Ahmad, Department of Microbiology, faculty of medicine, Sebha University,

Department of Microbiology, faculty of medicine, Sebha University,
Shamsi S Shamsi, Department of Plant Science, Microbiology Division, Research Laboratory, Sebha University

Department of Plant Science, Microbiology Division, Research Laboratory, Sebha University
Mohammed B Alsubbi, General Department, Faculty of Nursing, Sebha University, Libya

General Department, Faculty of Nursing, Sebha University, Libya
Ali A. Aldalouli, Department, PCR unit, clinical laboratory, Sebha Medical center.

Department, PCR unit, clinical laboratory, Sebha Medical center
Hamad A Almahdi, Department, PCR unit, clinical laboratory, Sebha Medical center

Department, PCR unit, clinical laboratory, Sebha Medical center
Alkeelani M M Alkeelani, Department, PCR unit, clinical laboratory, Sebha Medical center

Department, PCR unit, clinical laboratory, Sebha Medical center
Ibrahim A. Mohamed, Department of Biochemistry, faculty of medicine, Sebha University.

Department of Biochemistry, faculty of medicine, Sebha University.

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Published

2023-05-20

Issue

Vol. 18 No. 1 (2023): Exploring Diverse Research in Science and Healthcare: Insights from Our Latest Issue

Section

Original Research Article

License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

This article is published open access under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA 4.0). You may copy, redistribute, adapt and build upon it for non-commercial purposes, provided you credit the authors, cite the original publication in the Journal of Medical Sciences with its DOI, indicate any changes, and distribute any adaptations under the same licence. Copyright rests with the authors. For commercial reuse, please contact the corresponding author. Full terms: https://sebhau.edu.ly/journal/joms/copyright-licensing

How to Cite

Evaluation of four commercial COVID-19 diagnostic RT-PCR kits used in Libya. J Med Sci (Sebha) [Internet]. 2023 May 20 [cited 2026 Oct. 15];18(1):26-30. Available from: https://sebhau.edu.ly/journal/joms/article/view/2600