Detection of Haemoglobin S Among Libyan Children in Sebha Medical Centre
الكلمات المفتاحية:
Haemoglobin S، Sickle cell anaemia، Libyan childrenالملخص
Background: Sickle cell anaemia is caused by a single-base mutation leading to substitution of amino acid valine for glutamic acid at the sixth position of the β-globin chain. In the homozygous (Hb SS) both genes are abnormal, whereas in the heterozygous (Hb AS) only one chromosome carries the gene. Clinical presentation of sickle cell disease occurs frequently in children admitted in the Department of Paediatrics in Sebha Medical Centre.
Objective: The main aim of this study, was to apply the alkaline cellulose acetate electrophoresis technique to investigate the presence of haemoglobin S in blood collected from these children.
Materials and Methods: Blood samples were collected in EDTA anticoagulant tubes from 19 child (males = 13 and females = 6), suspected with sickle cell anaemia, and used for haematology and haemoglobin alkaline electrophoresis (pH 8.4) analysis.
Results: The haematological indices results were: total haemoglobin (X= 7.27 ± 1.35 g/dl), RBC (X= 2.56 ± 0.53 x 106/mm3), MCV (X= 88.74 ± 8.05 fl), MCH (X= 29.87 ± 2.40 pg) and Total leukocytes count (TLC) (X=13.69±6.31 x 106/mm3). The results of haemoglobin fractions as separated by alkaline electrophoresis were: 7 (36.8%) children had a haemoglobin fraction migrated in the position of Hb A, and 8 (42.1%) children had a single abnormal fraction migrated in the position of Hb S. Whereas, 4 (21.0%) children had two fractions migrated in the position of Hb A and S.
Conclusion: We conclude that a large prospective study would lead to better understanding of the distribution of these genetic disorder among Libyan populations, at least in this region of the country. Consequently, better genetic counselling and more efficient way of utilising the health care resources as well as improving family planning programmes could be achieved.
التنزيلات
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نُشر هذا المقال بالوصول المفتوح بموجب رخصة المشاع الإبداعي: نَسب المُصنَّف – غير تجاري – الترخيص بالمثل 4.0 دولي (CC BY-NC-SA 4.0). يجوز نسخه وإعادة توزيعه وتعديله والبناء عليه لأغراض غير تجارية، شريطة نسبته إلى مؤلفيه، والإشارة إلى نشره الأول في مجلة العلوم الطبية مع رقم المعرّف الرقمي (DOI)، وبيان أي تعديلات، وتوزيع أي عمل مشتق بموجب الرخصة نفسها. حقوق التأليف والنشر محفوظة للمؤلفين. لطلبات الاستخدام التجاري يُرجى التواصل مع المؤلف المراسل. الشروط الكاملة: https://sebhau.edu.ly/journal/joms/copyright-licensing
