A Comparative Evaluation of Stool Microscopy and Coproantigen ELISA in the Diagnosis of Cryptosporidiosis

المؤلفون

  • Arkaiya Mohammed Abdul Qader Department of Parasitology, Mansoura University, Egypt
  • Yosef Kubti Department of Parasitology, Benghazi University, Libya
  • A. H. Khan Department of Parasitology, Sebha University, Libya

الكلمات المفتاحية:

Cryptosporidiosis، Cryptosporidium، Coproantigen ELISA، Modified Ziehl-Neelsen stain، Auramine-rhodamine stain

الملخص

We evaluated the diagnostic performance of microscopical examination of staining techniques (modified Ziehl – Neelsen and Auramine – Rhodamine) and coproantigen enzyme linked immunosorbent assay (copro-ELISA) for cryptosporidiosis diagnosis. Copro-ELISA appeared to be most sensitive than staining techniques. The commercial copro-ELISA and Auramine - Rhodamine proved to be valuable diagnostic tools for Cryptosporidium infection.

التنزيلات

تنزيل البيانات ليس متاحًا بعد.

المراجع

Pillai D.R. and Kin K. (1999). Immunochromatographic strip-based detection of Entamoeba histolytica – E. dispar and Giardia lamblia coproantigen J. Clin. Microbiol. 37: 3017-3019.

Fayer, R. and Ungar, B.L.P. (1986). Cryptosporidium spp. and Cryptosporidiosis. Microbiol. Rev. 50:458-483.

Weber, R., Bryan, R.T., Bishop, H.S., Wahlquist, S.P., Sullivan, J.J. and Juranek, D.D. (1991). Threshold of detection of Cryptosporidium oocysts in human stool samples : evidence for low sensitivity of current diagnostic methods. J.Clin. Microbiol. 29:1223-1227.

Michel, M.Y., Khalifa, A.M. and Ibrahim, I.R. (2000). Detection of Cryptosporidium parvum antigen by coagulation test and ELISA East. Medtr, Health. J. 6:898-907.

Newman, R.D., Jaeger, K.L., Wunib, T. et al (1993). Evaluation of antigen capture enzyme linked immunosorbent assay for detection of Cryptosporidium oocysts. J. Clin. Microbiol. 31:2080-2084.

Marques, F.R., Cardoso, L.V., Cavasini, C.E. et al. (2005). Performance of immunoenzymatic assay for Cryptosporidium diagnosis of faecal samples. Brazilian J. Infect. Dis. 9:3-5.

El-Shazly, A.M., Atta M., Meniawy et al (2002). The use of Ziehl-Neelsen stain enzyme linked immunosorbent assay and nested polymerase chain reaction in diagnosis of cryptosporidiosis in immune competent-compromised patients. J. Egypt. Soc. Parasitol. 32:155-166.

Cheesbrough, M.(2004). Medical laboratory manual for tropical countries. Second edition. 217:179-187.

Garcia, L.S., Brucker, D.A., Brewer, T.C. and shimizu, R.Y. (1983). Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens, J.Clin. Microbiol. 18:185-190.

Paik, G.(1980) Regents, stains and miscallaneous test procedures. Am. Soc. Microbiol. D.C. 1022.

Arrowood, M.J. and Sterling, C.R. (1989). Comparison of convential staining methods and monoclonal antibody based methods for Cryptosporidium oocysts detection. J. Clin. Microbiol. 27:1490-1495.

Gomez Morales, M.A., Atzori, C., Ludovici, A., Rossi, P., Scaglia M. and Pozio, E. (1995). Opportunistic and non-oppotunistic parasites in HIV-positive and negative patients with diarrhea in Tanzania. Trop. Med. Parasital. 46:109-114.

التنزيلات

منشور

2012-01-01

إصدار

القسم

الأرشيف (النسخة الورقية)

كيفية الاقتباس

1.
A Comparative Evaluation of Stool Microscopy and Coproantigen ELISA in the Diagnosis of Cryptosporidiosis. J Med Sci (Sebha) [انترنت]. 1 يناير، 2012 [وثق 15 أكتوبر، 2026];11(1):48-50. موجود في: https://sebhau.edu.ly/journal/joms/article/view/5044

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