Determination of Caffeine and Some Other Drugs in Cappuccino, Nescafe, Cacao, Coffee Samples Collected from Some Libyan Markets Using High Performance Liquid Chromatography (RP-HPLC)
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Coffee Derivatives, Caffeine, Drugs, Extraction, Solvents, RP-HPLC
Abstract
This study aims to determine the caffeine and detection of some other drugs (which have commonly spread and caused suicides in the recent years in El-Bieda city, Libya) in cappuccino, Nescafe, cacao, and coffee samples using Reversed Phase - High Performance Liquid Chromatography (RP-HPLC). In the presented study, seventeen samples were collected from some Libyan markets. A rapid, simple, and reliable extraction method is developed and validated for the determination of caffeine and other drugs in the samples under study using dichloromethane, n-hexane, and methanol as the extracting solvents. The method is validated over a wide linear range of 2 – 10 µg/ml, with correlation coefficients being consistently greater than 0.999. The minimum caffeine level was observed in the Nesquik (Cacao) and Nestle Italiana S.P.A. samples (2.9129 µg/ml), while the Nestla (Nescafe) and Asbania samples showed the highest caffeine content (598.5315 µg/ml). The RP-HPLC measurements indicate that the results of caffeine concentrations in samples under study are in the range of 2.9129 - 598.5315 µg/ml with an average of 221.8630 µg/ml. The measurements by HPLC methods indicate that the samples under study are free from other drugs: olanzapine, diazepam, and alprazolam. The caffeine content in all the samples analyzed in this study is within the allowable limits set by the US Food and Drug Administration and documented values.
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References
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