Detection of Entamoeba Histolytica \E.Dipar and Ascaris Lumbricoides in Fresh Vegetables Consumed Collected Randomly Farms from Brack Al-Shati, Libya
Abstract
Vegetables are important for human health because they contain proteins, fibers, minerals, and vitamins. However, vegetables can be contaminated with enteric parasitic pathogens. This study aimed to investigate parasitic contamination with E.histolytica \E.dipar and Ascaris lumbriciodes of fresh vegetables consumed after washing with tap water collected randomly from farms in Brak al-Shati, Libya. Methods: The study focused on seven types of fresh vegetables: onion, carrot, turnip, lettuce, rocket, parsley, and chard. 10 samples of each type were checked without washing and after washing with tap water, approximately 100 g, washed with 250 ml of 0.95% NaCl, and shaken for 15 min. The vegetable was removed, and the remaining wash solution was left for 10 hours to sediment, the sediment examined follows: direct smear, iodine smear, 10x, 40x. Results: parasitic contamination with withE. histolytica\E.dispar was detected 15.7% (11/70), Ascaris lumbriciodes 22.8% (16/70); the percentage of contamination is mixed between E. histolytica\E.dispar and Ascaris it was 18.5% (13/70) after washing with tap water, 20% (14/70) Ascaris lumbriciodes, 14.3% (10/70) E.histolytica\ E. dispar, 10% (7/70) mixed.
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